牛疫ニ於ケル補體結合反應

第三報告:正常牛竝ニ牛疫牛淋巴腺ノ牛竝ニ家兎ニ對スル抗元性ニ就テ

抄録

Antigenic properties of lymphatic glands of normal and rinder-pest cattle were again studied by means of complement-fixation test. In this study, 4 hyperimmunized sera were used, namely, -(1) cattle serum against normal lymphatic glands, (2) cattle serum against pest lymphatic glands, (3) rabbit serum against normal lymphatic glands and (4) rabbit serum against pest lymphatic glands. The cross reaction between these sera and both raw and boiled extracts of normal and pest lymphatic glands wese tested. The results sbtained are summarized as follows:
(1) Sera of rabbits and cattle which had been hyperim-munized with normal lymphatic glands, reacted positively with raw extract of either normal or pest lymphatic glands. These sera having been used, there was no difference observed in antigenic property between normal and pest lymphatic glands. The extracts, when boiled at 100°C, however, lose their antigenic action.
(2) The reaction caused by the immune cattle serum belongs to an immune iso-reaction. The question as to whether it exhibits a strict organ specificity excluding the species specificity entirely, however, remains still undetermined. But, judging from the fact that all the antigens tested showed no decided individual differ-ences in their activity, individual specificity may be excluded from consideration.
In this reaction, the cattle immune serum does not lose its activity, when it is heated at 55°C.
(3) As it was shown in previous papers, serum of cattle, hyperimmunized with pest lymphatic glands, reacts specifically with boiled extract of pest lymphatic glands, while it does not react with boiled extract of normal lymphatic glands.
The serum of rabbit, hyperimmunized with the same material, on the contrary, fails to react specifically with pest lymphatic glands. So it behaves quite similarly to the serum obtained by hyperimmunization with normal lymphatic glands. It is very inter-esting that the pest lymphatic gland can produce the pest-specific antibody in cattle while it fails to do so in rabbit.
(4) It is presumed that the reaction between raw extract of pest lymphatic glands and its homologous cattle serum may be a complex of 2 simple reactions, namely, the one specific to rinder-rest and the other specific to tissue antigen. Though the author did not yet succeed in separating the 2 antigens from the extract and the 2 antibodies from the immune serum, such a presumption is justified when the reactions in question are quantitatively studied.