Abstract
It is well known that some bacteria, as Shiga's dysentery bacillus, Fraenkel's pneumococcus and bacillus of the rabbit pneumonia, posses the heterogeneous antigenic property.
I have also proved that a strain of Bac. bovisepticus and one out of 3 strains of Bac. lepisepticus, which isolated from rabbit septicaemia by me, have the same property.
The following is the conclusion of my study.
1) Both Bac. bovisepticus anc Bac. lepisepticus were proved to have a property to unite with a known heterogeneous antibody contained in a rabbit immune-serum against guinea-pig kidney.
2) Both of the bacilli produced anti-sheep haemolysin, when injected into rabbit.
3) The haemolysin was capable of haemolyzing the red corpuscles of sheep and goat, but not those of man, cow, horse, dog, swine, rabbit, guinea-pig, rat, mouse and frog.
4) The haemolysin was absorbed by a watery suspension or alcoholic extract of guinea-pig kidney.
5) The anti-bovisepticus and anti-lepisepticus serum from the immunized rabbits showed complemement fixation and precipitin reaction with the alcoholic extract of guinea-pig kidney,
6) The heterogeneous antibodies in the anti-bovisepticus and anti-lepisepticus serum were proved to be quite different from the specific antibodies, agglutinin, precipitin and complement fixing substance, which were also demonstrated in the serum.
7) Each of the rabbit serum immunized against guinea-pig kidney, sheep's red corpuscles, Bac. bovisepticus and Bac. lepisepticus respectively was proved to have a toxicity for guinea-pig. No parallelism was shown between the haemolytic titer and the toxicity.
8) The reaction of complement fixation and precipitation with the above sera, whereby an alcoholic extract of guinea-pig kidney being used as antigen, was not found to be parallel in degree to its toxicity for guinea-pig in a strict sense.
