[in Japanese]

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It is well known that some bacteria, as Shiga's dysentery bacillus, Fraenkel's pneumococcus and bacillus of the rabbit pneumonia, posses the heterogeneous antigenic property.
I have also proved that a strain of Bac. bovisepticus and one out of 3 strains of Bac. lepisepticus, which isolated from rabbit septicaemia by me, have the same property.
The following is the conclusion of my study.
1) Both Bac. bovisepticus anc Bac. lepisepticus were proved to have a property to unite with a known heterogeneous antibody contained in a rabbit immune-serum against guinea-pig kidney.
2) Both of the bacilli produced anti-sheep haemolysin, when injected into rabbit.
3) The haemolysin was capable of haemolyzing the red corpuscles of sheep and goat, but not those of man, cow, horse, dog, swine, rabbit, guinea-pig, rat, mouse and frog.
4) The haemolysin was absorbed by a watery suspension or alcoholic extract of guinea-pig kidney.
5) The anti-bovisepticus and anti-lepisepticus serum from the immunized rabbits showed complemement fixation and precipitin reaction with the alcoholic extract of guinea-pig kidney,
6) The heterogeneous antibodies in the anti-bovisepticus and anti-lepisepticus serum were proved to be quite different from the specific antibodies, agglutinin, precipitin and complement fixing substance, which were also demonstrated in the serum.
7) Each of the rabbit serum immunized against guinea-pig kidney, sheep's red corpuscles, Bac. bovisepticus and Bac. lepisepticus respectively was proved to have a toxicity for guinea-pig. No parallelism was shown between the haemolytic titer and the toxicity.
8) The reaction of complement fixation and precipitation with the above sera, whereby an alcoholic extract of guinea-pig kidney being used as antigen, was not found to be parallel in degree to its toxicity for guinea-pig in a strict sense.

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[in Japanese]

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The trypanosome employed for the present experiment, in all probability identical with T. evansi, is the strain isolated from a water-buffalo in Formosa. The animals, such as cattle, horses and rabbits, were inoculated with this trypanosome and their sera were examined weekly after isoculation for a long period by the Wassermann (Browning) and Sachs-Georgi tests. The results of both syphilis reactions obtained in these experimentally infected animals are summarized as follows:
1) Of 8 typically infected Korean calves, all showed uniformly a strikingly positive reaction in the Wassermann complement-fixation and Sachs-Georgi flocculation tests. The vacillation of reaction was noted in all cases and ran almost parallel in both tests (Tables I-IV).
2) The Wassermann reaction in the infected cattle occurs generally some 10 weeks after inoculation. The positive reaction so developed, however, continues for some period and disappears. If the patient takes a delayed course, such a positive attack is repeated at irregular intervals. In the positive reaction extended for a long duration, there is also observed a number of oscillations in the complement-fixing power.
3) The Sachs-Georgi reaction, as compared with the Wassermann, appears far earlier and turns positive, in general 1 week after inoculation. The duration of positive reaction here is decidedly longer than that of complement-fixation. In the positive Sachs-Georgi reaction when long continued, the fluctuation is also noted to certain degree, though not so distinctly as in case of the Wassermann.
4) Two Korean calves in the suspected latent infection yielded a slight and unsteady positive effect in the complement-fixation test, while they exhibited a continued positive reaction in the flocculation test (Nos. 5 and 6, Table III). Such a dissociation of 2 reactions, therefore, may probably ascertain that the tested cattle contracts a latent infection with the trypanosome.
5) If it is to diagnose the trypanosomal infection of cattle by either of the 2 syphilis tests, we prefer the Sachs-Georgi test rather to the Wassermann, on account of the reasons mentioned above. However, the systematically repeated studies of the reaction only permit us to trace the bovine trypanosomiasis in all cases.
6) The serum from normal cattle frequently showed the presence of reacting substances in the Wassermann test (13 in 50), but it is exceptional in the Sachs-Georgi test (1 in 50) (Tables V). The case reacted negatively in 2 tests may later exhibit a temporary positive turn, when the test is repeatedly carried on (Table VI).
7) In general the reaction of normal sera develops slightly and disappears after 1 or 2 weeks. This feature contrasts markedly with the high degree of complement-fixation and the long duration of flocculation elicited with sera from cases of active trypanosomiasis.
8) Contrary to the case of infected cattle, out of 9 Korean ponies infected with T. evansi, 4 seemed to react negatively and 3 weakly (Tables VII anp VIII), while one of the remaining 2 gave a strongly positive result in both reactions and the other in the flocculation only (Table IX).
9) A Korean pony inoculated with T. equiperdum reacted slightly in both tests (Table X).
10) One of 5 normal ponies, each being tested 2-7 times, showed a weakly positive effect once in respective syphilitic reactions (Table X).
11) Of 10 trypanosome-infected rabbits, 7 brought a decidedly positive result in both reactions, the other 3 reacting weakly. The 2 reactions both turn positive in about 3 weeks after inoculation and fluctuate nearly parallel with sera from the same individual (Table XI).
12) The serum from normal rabbits frequently reacts positively in one or both of 2 tests.

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