Abstract
Two antigenic substances (precipitating lines) in the crude antigen of adult flukes of Fasciola sp., as demonstrated by the agar-gel diffusion reaction, were isolated individually from ten agar-gel plates and injected into rabbits to produce antisera against such pure antigens. The production of mono-specific antisera for each of these two antigenic substances could be evidenced by an agar-gel immunodiffusion assay. Indirect immunofluorescent studies on the adult fluke with these mono-specific antisera demonstrated that of the two antigenic substances, one was distributed mainly in the subcuticular muscle layer and subcuticular cells (cells lying under the subcuticular muscle layer are called "subcuticular cells" in the present paper) and the other localized in the excretory ducts of the fluke.
