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Volume 41 (1979)
- Issue 6 Pages 583-
- Issue 5 Pages 471-
- Issue 4 Pages 351-
- Issue 3 Pages 227-
- Issue 2 Pages 97-
- Issue 1 Pages 1-
Volume 41, Issue 3
Displaying 1-12 of 12 articles from this issue
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Shinobu YOSHIHARA, Kyo SUZUKI, Tsuneji SUTO, Jinkichi FUJITA1979 Volume 41 Issue 3 Pages 227-233
Published: June 25, 1979
Released on J-STAGE: February 13, 2008
JOURNAL FREE ACCESSTwo antigenic substances (precipitating lines) in the crude antigen of adult flukes of Fasciola sp., as demonstrated by the agar-gel diffusion reaction, were isolated individually from ten agar-gel plates and injected into rabbits to produce antisera against such pure antigens. The production of mono-specific antisera for each of these two antigenic substances could be evidenced by an agar-gel immunodiffusion assay. Indirect immunofluorescent studies on the adult fluke with these mono-specific antisera demonstrated that of the two antigenic substances, one was distributed mainly in the subcuticular muscle layer and subcuticular cells (cells lying under the subcuticular muscle layer are called "subcuticular cells" in the present paper) and the other localized in the excretory ducts of the fluke.View full abstractDownload PDF (767K) -
Katsuya ISHIHARA, Akira HASHIMOTO, Misao ONUMA, Takeshi MIKAMI, Tsuyos ...1979 Volume 41 Issue 3 Pages 235-243
Published: June 25, 1979
Released on J-STAGE: February 13, 2008
JOURNAL FREE ACCESSSerological and hematological tests were performed in parallel at 3 to 6-month intervals over a time course on 178 apparantly normal Japanese Black cattle raised in T Village of H region in Japan. In this village bovine leukemia had been identified repeatedly. As a result, 60.7% of the cattle was serologically positive in the immunodiffusion test (ID) and/or complement fixation test. The percentage of the cattle positive in ID was significantly higher after the pasture period than before it. When the results of the hematoloical tests were determined on the basis of the criteria for Japanese Black cattle, 193 cattle were divided into three groups, Group 1 of negative test, 83.4%, Group 2 of suspitious test, 13.0%, and Group 3 of persistent lymphocytosis, 3.6%. The positive percentage determined by one or three rounds of hematological testing was generally lower than that by serological testing. A low correlation was observed in the serologically positive ratio among the three groups classified hematologically from examinations over a time course. The lymphocyte count was significantly higher in cattle after becoming ID-positive than in the same cattle when they were ID-negative. The rate of leukemic onset during 26 months of observation was 3.0% among the serologically positive cattle and 28.6% among the hematologically positive cattle classified into Group 3.View full abstractDownload PDF (870K) -
Kentaro YOSHIMURA, Hiroko AIBA, Nakami HIRAYAMA, Toshihide H. YOSIDA1979 Volume 41 Issue 3 Pages 245-257,259
Published: June 25, 1979
Released on J-STAGE: February 13, 2008
JOURNAL FREE ACCESSThe occurrence of acquired resistance and the kinetics of humoral immune responses to Angiostrongylus cantonensis infection were studied in eight inbred rat strains. Consequently, it was found that both BUF and ACI strains acquired less protective immunity than other strains and, conversely, Tokyo and NIG strains yielded higher resistance. The remaining strains showed various intermediate degrees of acquired resistance. Striking strain differences in pattern of reagin production were found; Tokyo was a high responder while ACI was a low responder. In contrast, the kinetics of indirect hemagglutination (IHA) antibody response was essentially the same in all the strains examined. The IHA antibody titers were generally high in both Tokyo and NIG. Histopathologically, ACI was distinct from NIG in that fibrotic reactions around the nodules of developing eggs in the lung parenchyma were almost undiscernible. These data suggest that genetic factors would be involved in acquired resistance and reaginic antibody responses to A. cantonensis infection in rats.View full abstractDownload PDF (1390K) -
Naotaka ISHIGURO, Gihei SATO, Kouji TAKEUCHI, Akira NAKAYAMA1979 Volume 41 Issue 3 Pages 261-272
Published: June 25, 1979
Released on J-STAGE: February 13, 2008
JOURNAL FREE ACCESSThe distribution of Salmonella on a pig farm and the mode of spreading of Salmonella infection were studied for 2 years. The possibilities of exotic fresh infection and cross-infection within the farm were investigated by dividing serovars of Salmonella isolates or biovars of S. typhimurium. During the present investigation, 745 (22.0%) out of 3, 374 samples of pen fecal samples and various environmental specimens examined were positive for Salmonella. The serovar strains isolated were S. typhimurium 582, S. anatum 153, S. senftenberg 65, S. livingstone 7 and S. infantis 6. Of the 5 serovars S. typhimurium was persistently found for 2 years. These isolates were classified into 2 different biovars (3 and 19, and 1 and 2) according to Duguid's primary tests and the method of Brandis, respectively. Furthermore, 11 different sub-biovars were found within 2 different biovar by Duguid's secondary tests. Appearance of different serovars and the change of biovars in S. typhimurium indicated the occurrences of different exotic infection sources on this farm. The isolation rate of Salmonella from manure and sewage samples and pen fecal samples of market pigs indicated that this farm was contaminated heavily with Salmonella. Antibiotic susceptibility of 317 isolates of the 5 Salmonella serovars from pig pen fecal samples and 163 isolates from various environments was examined. Over 50% of S. typhimurium isolates tested and each one of S. anatum and S. senftenberg were resistant to TC, SM and SA. However, the antibiogram was not useful as an epidemiological marker on this farm.View full abstractDownload PDF (1020K) -
Takamasa SHIMIZU, Kazuhiko NUMANO, Koji UCHIDA1979 Volume 41 Issue 3 Pages 273-282
Published: June 25, 1979
Released on J-STAGE: February 13, 2008
JOURNAL FREE ACCESSA total of 850 birds belonging to 40 species within 13 orders were examined for mycoplasmas during 1968 through 1977. In addition, 2, 848 dead-in-shell chicks, 275 pathological specimens and 285 normal semen samples of chickens were subjected to the study. It was revealed that birds belonging to either Galliformes or Columbiformes harbored mycoplasmas in high incidence. Serological identification of 440 isolates proved that both Mycoplasma gallisepticum and M. synoviae parasitized among chickens, with an exception of sparrows. Host range of M. gallinarum was the widest of all species or serogroups of avian mycoplasmas. M. iners and serogroups C, D, and I-J-K-N-Q-R were detected from various tissues and sperm samples of domesticated and wild birds belonging to Galliformes. Both M. columbinum and M. columborale were detected only from pigeons, which were considered as the common hosts for these two species of mycoplasmas.View full abstractDownload PDF (907K) -
Hajime MATSUHASHI1979 Volume 41 Issue 3 Pages 283-289,298
Published: June 25, 1979
Released on J-STAGE: February 13, 2008
JOURNAL FREE ACCESSLight and electron microscopic studies were made on the renal juxtaglomerular cells (JGC) in mice maintained without water supply for one, three or seven days. The index of granularity (Hartroft and Hartroft, 1953) was 49.0, 24.7 and 48.4 on the average, respectively. On the third day of experiment, mature juxtaglomerular cell granules (JGG) decreased in number, and Golgi apparatus was well developed. Near the Golgi region, round or rhomboidal granular structures with a crystalline pattern were found. JGC seemed to be active in forming and releasing JGG. On the seventh day, the feature of JGC differed apparently from that found on the third day. Some JGG tended to fuse into a single large granule with an irregular contour, sometimes having low density. It seems that JGG may have presented a distinct lysosomal nature.View full abstractDownload PDF (3034K) -
Wataru SHIRAI, Shuichi KURE, Tsutae KASHIMA, Tsuneo SATO, Takayuki SAI ...1979 Volume 41 Issue 3 Pages 299-306
Published: June 25, 1979
Released on J-STAGE: February 13, 2008
JOURNAL FREE ACCESSA study was conducted to investigate the distribution and morphology of brilliant cells in the bovine normal uterus and uterus suffering from various types of endometritis, and to clarify the relationship between these cells and mast cells. Uterine specimens were collected from 52 cows at the Shibaura Slaughterhouse. They consisted of 15 normal ones, 21 with chronic catarrhal endometritis, 9 with chronic purulent endometritis (4 mild and 5 severe), and 7 with gangrenous endometritis. The results obtained are as follows. 1) Brilliant cells were distributed in the normal uterus in the same manner as in the uterus affected with endometritis. They were the most numerous in the stratum vasculare, which was followed by the stratum basale and the tunica muscularis in the order listed. They were hardly noticed in this stratum functionale and the tunica serosa. 2) These was a difference in the number of brilliant cells between the normal and affected specimens. These cells were the most abundant in the specimen with chronic catarrhal endometritis. They were reduced in number in specimens with severe chronic purulent endometritis and gangrenous endometritis. 3) Brilliant cells multiplied by mitosis and amitosis. 4) Brilliant cells had granules which were eosinophilic and which showed orthochromasia by toluidine blue staining. They were positive for periodic acid-Schiff reaction. From these results, it was concluded that some of the brilliant cells possessed essentially the same tinctorial characteristics as the type-1 mast cell of Riley and that of Nakashima and Hiekawa.View full abstractDownload PDF (1940K) -
Akio SHIGA, Kenichi SHINOZAKI1979 Volume 41 Issue 3 Pages 307-318
Published: June 25, 1979
Released on J-STAGE: February 13, 2008
JOURNAL FREE ACCESSA balance experiment was conducted on five ewes divided into two groups (young and old) which had been fed on two control and four low-magnesium diets of various levels of Mg. Decreased dietary Mg resulted in linear decreases in fecal, urinary and serum Mg in both groups (P<0.01 and <0.001). With regression equations, minimum dietary requirements, true absorption values of Mg and endogenous fecal Mg in these young and old groups were estimated at 11.78 and 14.50 mg/kg body wt/day, 41.7 and 39.8%, 3.11 and 3.46 mg/kg body wt/day, respectively. Exponential regressions were obtained between serum and urinary Mg in both groups (P<0.001). Significant positive correlations of Mg with Ca in Mg deficiency were found in urine and serum (P<0.05, <0.01 and <0.001), and acute lowering of serum Mg concentration was accompanied with hypocalcemia in both groups. Mobilization from body pools of Mg in deficiency was about 1.1 mg/kg body wt/day and there was observed no difference by age in Mg mobilization in both groups. The lowering of serum Mg concentration more remarkable in old group in Mg deficiency was presumedly due to the hypofunctional Mg absorption in intestines and Mg excretion in kidneys in old group. With Mg intake of 2.9 mg/kg body wt/day toward seventh day, an ewe in old group developed tetany, which was almost coincident with the increase in serum Ca/Mg ratio due to the relatively high rise of serum Ca concentration. At the tenth day serum Ca/Mg ratio increased up to 16.7.View full abstractDownload PDF (1089K) -
Koichi HARUTA, Masanori SASAKI, Yuko MIYAZONO, Shichiro INADA, Shigeru ...1979 Volume 41 Issue 3 Pages 319-323
Published: June 25, 1979
Released on J-STAGE: February 13, 2008
JOURNAL FREE ACCESS -
Masato ABE, Hiroomi ONO1979 Volume 41 Issue 3 Pages 325-329,331
Published: June 25, 1979
Released on J-STAGE: February 13, 2008
JOURNAL FREE ACCESS -
Shoichiro FUJITA, Ei-ichi KOKUE, Toyoaki HAYAMA1979 Volume 41 Issue 3 Pages 333-335,337
Published: June 25, 1979
Released on J-STAGE: February 13, 2008
JOURNAL FREE ACCESS -
Toshio WADA, Sumio HANDA, Shiro MOHRI1979 Volume 41 Issue 3 Pages 339-341
Published: June 25, 1979
Released on J-STAGE: February 13, 2008
JOURNAL FREE ACCESS
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