The Japanese Journal of Veterinary Science
Online ISSN : 1881-1442
Print ISSN : 0021-5295
ISSN-L : 0021-5295
Selective Isolation of Mycoplasma hyosynoviae by Anaerobic Cultivation
Manabu OGATASeiji KAWAMURAKoshi YAMAMOTO
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1982 Volume 44 Issue 5 Pages 725-731,733

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Abstract
Growth of type strains of 23 mycoplasma species as well as field strains of M. hyorhinis was compared in aerobic and strict anaerobic cultivation. In anaerobic cultivation, the growth of five species including M. hyorhinis was suppressed, whereas that of other four species including M. hyosynoviae was enhanced. The remaining 14species showed similar growth in the two atmospheric conditions. While all anaerobically enhanced species belonged to arginine hydrolyzing mycoplasma group, anaerobically suppressed species were all arginine-non-hydrolyzers. In order to inhibit the growth of M. hyorhinis, rapid attainment of strict anaerobic condition was required. In a field survey, the anaerobic cultivation was shown to be effective for selective isolation of M. hyosynoviae from nasal cavities of pigs.
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© The Japanese Society of Veterinary Science
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