Tumor-Associated Antigen Detected by Complement-Dependent Antibody Cytotoxicity Test and Immunofluorescence Test in Enzootic Bovine Lymphosarcoma

Abstract

Tumor-associated antigens were studied on the neoplastic cells of cattle affected with bovine lymphosarcoma (BL) by complement-dependent antibody cytotoxicity (CDAC) test and immunofluorescence (IF) test using antiserum produced in rabbits which were immunized with tumors from four cows. The antiserum from rabbits was absorbed with erythrocytes and pooled cells of lymph nodes from normal cows. Neoplastic cells from 18 of 21 cases affected with enzootic BL (EBL) showed cytotoxic reaction by CDAC test, but those were negative from one case of calf type BL (CBL). Cytoplasmic antigen was detected by IF test in acetone-fixed neoplastic cells from all 52 EBL cows examined. No reaction was observed in lymphocytes of normal 9 cows and 3 fetuses from EBL cows as well as in neoplastic cells of 3 CBL calves. Fluorescence was observed in 37% to 97% of peripheral blood lymphocytes (PBL) from all EBL cases examined. None of PBL from 7 normal cows showed positive reaction.