Case Report on Mixed Infection of Canine Parvovirus and Canine Coronavirus : Electron Microscopy and Recovery of Canine Coronavirus

Abstract

Occurrence of coronavirus-like virions and associated degenerative changes were seen in the enterocytes of jejunal villi of a mongrel dog which had been diagnosed first as canine parvoviral disease from virological and pathological examinations. A cytopathic agent was isolated in dog kidney cell cultures from intestinal contents of the present case. The isolate, Toda strain, had morphological and physicochemical properties in common with the viruses of the coronavirus group. Toda strain was neutralized by ascites obtained from a cat with feline infectious peritonitis. Thus the case was diagnosed precisely as the mixed infection of canine parvovirus and canine coronavirus.