Abstract
A viral agent producing cytopathic effect of syncytial type and intranuclear inclusion body was isolated from swine testicle (ST) cell culture. It could be passaged in a culture of porcine lung macrophage, but with the infected cell culture fluid it could not be passaged in ST cell culture. The isolate was identified as porcine cytomegalovirus (PCMV) on the basis of the following results: 1) the virus had the same antigenicity as the B6 strain of PCMV by fluorescent antibody test, 2) morphology of the virus particle by electron microscopy was similar to that of herpersvirus, and 3) the virus produced the same lesions as PCMV-associated illness in gnotobiotic piglet.
