-
Osamu KAJIKAWA, Yukio YAGI, Hiroyuki KOYAMA, Hiroshi SAITO
1985 Volume 47 Issue 5 Pages
683-690
Published: October 15, 1985
Released on J-STAGE: February 13, 2008
JOURNAL
FREE ACCESS
Three monoclonal antibodies against Theileria sergenti were prepared and used in indirect fluorescent antibody staining procedure to determine the percentages of infected erythrocytes in a calf under parasitemia for 21 days. The percentages detected by the monoclonal antibodies were slightly lower than those by bovine immune serum or Giemsa stain. A blocking test showed that the three monoclonal antibodies recognize the same antigenic determinant of T.sergenti. Another blocking test also indicated that the monoclonal antibodies recognize one of the antigenic determinants on the organism detected by polyclonal antisera. However, the shapes of T.sergenti stained by the monoclonal antibodies were similar to that stained by Giemsa stain. The immunoglobulin subclass of these monoclonal antibodies was IgG
1, κ light chain.
View full abstract
-
Hitoshi KITAGAWA, Yoshihide SASAKI, Katsuya ISHIHARA
1985 Volume 47 Issue 5 Pages
691-696
Published: October 15, 1985
Released on J-STAGE: February 13, 2008
JOURNAL
FREE ACCESS
Central venous pressure (CVP) was measured before and immediately after surgical heartworm removal from the jugular vein in 22 dogs with dirofilarial hemoglobinuria (caval syndrome). In 19 cases which recovered by sugical removal, CVP was considerably high from 32 mmH
2O to 187 mmH
2O with a mean of 101.6±35.1(S.D.)mmH
2O before heartworm removal. CVP fell in all cases immediately after heartworm removal. The mean CVP after heartworm removal was 61.0±42.9 mmH
2O. This level was significantly lower than that before heartworm removal (p<0.001). After heartworm removal, systolic cardiac murmur and jugular pulse improved and hemoglobinemia and hemoglobinuria disappeared. Two of 3 cases, which died after heartworm removal, indicated a high CVP over 200 mmH
2O and showed the signs of severe circulatory failure (right heart failure). They also displays terminal signs such as prostration and low body temperature. After heartworm removval, systolic murmur improved passably, but high CVP levels over 175 mmH
2Oremained in both cases. Another case did not indicate a high CVP level (66 mmH
2O) before heartworm removal. Forty-seven heartworms were removed from this case. However, there was no recovery of the systolic murmur, and CVP fell only 5 mmH
2O. In this case, the knotting of a heartworm around the tricuspid chordae was confirmed by autopsy. It was considered that the existence of heartworm mass at the tricuspid valve orifice would be the cause of the high CVP level in this disease. And it was suggested that the tricuspid insufficiency by heartworm mass at the tricuspid valve orifice would be the direct cause of acute circulatory insufficiency that indicates the onset of dirofilarial hemoglobinuria together with many symptoms.
View full abstract
-
Junsuke SHIRAI, Minoru NARITA, Yuji IIJIMA, Hitoshi KAWAMURA
1985 Volume 47 Issue 5 Pages
697-703
Published: October 15, 1985
Released on J-STAGE: February 13, 2008
JOURNAL
FREE ACCESS
A viral agent producing cytopathic effect of syncytial type and intranuclear inclusion body was isolated from swine testicle (ST) cell culture. It could be passaged in a culture of porcine lung macrophage, but with the infected cell culture fluid it could not be passaged in ST cell culture. The isolate was identified as porcine cytomegalovirus (PCMV) on the basis of the following results: 1) the virus had the same antigenicity as the B6 strain of PCMV by fluorescent antibody test, 2) morphology of the virus particle by electron microscopy was similar to that of herpersvirus, and 3) the virus produced the same lesions as PCMV-associated illness in gnotobiotic piglet.
View full abstract
-
Yuji TAKAHASHI, Yasumasa KIDO, Masayuki NAOI, Ei-ichi KOKUE
1985 Volume 47 Issue 5 Pages
705-709
Published: October 15, 1985
Released on J-STAGE: February 13, 2008
JOURNAL
FREE ACCESS
Usability of a repeated renal biopsy was examined for the estimation of drug residue in the pig kidneys. The renal biopsy was carried out under laparoscopic observation. Eleven castrated pigs were given intramuscularly 1 mg (potency) of gentamicin (GM) per kg body weight. The renal parenchyma was sampled by the biopsy 3, 10, 20, 30, 40 and 50 days after administration. Two pigs were sacrificed to obtain the kidneys immediately after each biopsy. No clinical manifestations were observed except transient hematuria in all biopsied pigs. The mean concentration of GM in the biopsied samples decreased similarly to that in the kidneys of sacrificed pigs. There was no significant difference in the analytical values between the biopsied samples and those obtained by sacrifice immediately after the biopsies. These results suggest that the estimation of drug residue in the pig kidneys by the repeated biopsy is useful to determine the withdrawal time of drugs remaining predominantly in the kidneys.
View full abstract
-
Shoko SUZUKI, Akio FUKUSHO, Yasuo MIURA, Jaime Arias IBARRONDO, Junsuk ...
1985 Volume 47 Issue 5 Pages
711-717
Published: October 15, 1985
Released on J-STAGE: February 13, 2008
JOURNAL
FREE ACCESS
A cytopathic virus was isolated from the primary kidney cell cultures of apparently healthy guinea pigs reproduced in Mexico between 1982 and 1983. This virus produced intranuclear type A inclusion bodies in the infected cell cultures. Viral replication was inhibited by IUdR, indicating that the viral nucleic acid was DNA. The virus was sensitive to ether, chloroform and acid (pH 3.0), and passed readily through a 200 nm filter, but not through a 100 nm filter. Electron microscopy showed a spherical particle with envelope. These properties were consistent with those of Herpesviridae. In serologic survey of guinea pigs in Mexico and Japan, and of mice, hamsters, rats, rabbits, pigs, goats, horses and calves in Japan, neutralizing antibody against the isolate GPM 83-11 was detected in 4 of 46 infant guinea pigs about 3-4 weeks of age and in 9 of 30 breeding guinea pigs reproduced in Mexico, but neither in any of guinea pigs nor other animals in Japan.
View full abstract
-
Eri OGAWA, Fumiaki AKAHORI, Kosaku KOBAYASHI
1985 Volume 47 Issue 5 Pages
719-729
Published: October 15, 1985
Released on J-STAGE: February 13, 2008
JOURNAL
FREE ACCESS
The hemolytic action of the onion extract against canine erythrocytes was investigated. Onion-exposed erythrocyte suspension or hemolysate underwent rapid oxidation of hemoglobin to methemoglobin and subsequent denaturation leading to the formation of Heinz bodies. During the process of hemoglobin breakdown, the presence of superoxide radical (O
-2) was demonstrated by using co-oxidation of epinephrine to adrenochrome. Carboxyhemoglobin was resistant to oxidation by the onion extract, and there was no liberation of superoxide. Superoxide dismutase (SOD), catalase, reduced glutathione (GSH) or mannitol had no effect on hemoglobin oxidation, suggesting that activated oxygens, O
-2, H
2O
2 and ·OH were not involved in oxidation of the heme iron (Fe
2+). Oxyhemoglobin is deduced to be a source of both methemoglobin and O
-2, and their production which is accelerated by the onion extract is assumed to be a primary step of erythrocyte breakdown. Osmotic fragility of the red cell membrane increased markedly during exposure to the onion extract, and significant inhibition by SOD and catalase manifested that O
-2 and its derivative H
2O
2 were both responsible for the damage of the cell membrane.
View full abstract
-
Noboru MIURA, Nobuo SASAKI, Hiroyuki OGAWA, Akira TAKEUCHI
1985 Volume 47 Issue 5 Pages
731-739
Published: October 15, 1985
Released on J-STAGE: February 13, 2008
JOURNAL
FREE ACCESS
Six male beagle dogs were administered estradiol benzoate (E
2) for 30 days at a dose of 0.1 mg/kg every day or 0.3 mg/kg every 3rd day and changes in hematology, bone marrow cells and the blood coagulation were investigated. Bleeding tendencies with severe thrombocytopenia occurred 15 or 18 days after the first E
2 injection. Three of 6 dogs died of the intra-thoracic hemorrhage on days 21, 33 and 45 respectively. White blood cells first increased to 4-10×10
4/mm
3 on days 15-21, then decreased thereafter. Red blood cells first decreased and then slowly increased after days 21-27. Platelets increased transiently soon after the E
2 administration, but then decreased rapidly and were below 3000/mm
3 on day 15. After day 24, platelets again increased. By the bone marrow examinations, myeloid cells increased and erythroid cells decreased considerably on days 15-18. After day 24, myeloid cells decreased and erythroid cells increased and the total number of marrow cells decreased at the end of the experiment. There were no abnormal findings in the coagulation examinations in all the dogs.
View full abstract
-
Hideharu SASAKI, Takuma HANAKI, Osamu ITOH, Tsuneyoshi OMORI, Minoru M ...
1985 Volume 47 Issue 5 Pages
741-748
Published: October 15, 1985
Released on J-STAGE: February 13, 2008
JOURNAL
FREE ACCESS
Fresh bovine sera absorbed with sheep red blood cells (RBC) agglutinated RBC weakly sensitized with anti-RBC rabbit antibody, indicating the sera to be satisfactory as the source of conglutinating complement. Conglutinin was present in excess in these sera. Based on these observations we developed a conglutinating complement absorption (CCA) test using the conglutinating system of fresh bovine serum and sensitized RBC. Inactivated bovine sera were found to inhibit or enhance bovine complement and also to agglutinate normal RBC. These activities were readily eliminated by treating the sera with bovine complement and anti-RBC rabbit serum and then absorbing with RBC. This treatment was shown to be prerequisite for the bovine serum to be assayed by the CCA test. The CCA test was proven to be useful and practical for the assay of brucella antibody in bovine sera. The test was shown to be as sensitive and specific as the hemolytic complement fixation test. One of the advantages of the new CCA test is that the results can be readily read by the sedimentation pattern of RBC, whereas in the current CCA test the results are read by a rather cumbersome procedure in which the cells are centrifuged and briskly resuspended to distinguish strong conglutination from non-specific weak agglutination. The new CCA test was therefore readily adapted to the microtiter method. The method gave antibody titers agreeing well with those obtained by the tube method.
View full abstract
-
Haruhisa TAKAMATSU, Fusaoki KOIDE
1985 Volume 47 Issue 5 Pages
749-759
Published: October 15, 1985
Released on J-STAGE: February 13, 2008
JOURNAL
FREE ACCESS
Porcine spontaneous cell-mediated cytotoxicity (SCC) was recognized in spleen and peripheral blood lymphocytes, and the cytotoxicity of spleen mononuclear lymphocytes were higher than that of peripheral blood mononuclear cells against K-562 human myeloid cell. Scc was augmented spontaneously when incubated lymphocytes at 37°C with 10% fetal calf serum containing medium or serum free media, however, their augmentation of SCC was reduced by autologous and homologous serum. The maximum augmentation of SCC by incubation was achieved at 40 hr, the kinetics was correlated with the DNA synthesis of whole mononuclear lymphocytes. When adherent cells (ADC) were removed from whole peripheral mononuclear cells (WPBL) or whole spleen mononuclear lymphocytes (WSL), SCC was not augmented by incubation at 37°C. Addition of cultured fluid from WPBL or WSL on non-adherent lymhocyte (NAC) culture, augmentation of SCC by incubation was also recognized. IL-1 and IL-2 like activity was detected in these cultured fluids from WPBL and WSL. Porcine SCC was also boosted in vitro by incubation with IFN-γ inducer OK-432. This augmentation was not dependent on ADC. These results suggested that spontaneous augmentation of porcine SCC is dependent on the formation of IL-1 by ADC and IL-2 by NAC. Also IFN-γ may play an important role of SCC augmentation in vitro.
View full abstract
-
Noboru FUJIOKA, Kosaku FUJIWARA, Kazuya YAMANOUCHI, Masanori HAYAMI
1985 Volume 47 Issue 5 Pages
761-768
Published: October 15, 1985
Released on J-STAGE: February 13, 2008
JOURNAL
FREE ACCESS
Histopathological and hematological changes in acute infection with mouse hepatitis virus-3 (MHV-3) were examined in three strains of mice with different susceptibility to the virus. After intraperitoneal injection with MHV-3, CDF1 (BALB/c ×DBA/2) mice survived while BALB heterozygous (nu/+) and nude (nu/nu) mice died showing severe hepatitis. Degenerative changes including necrosis were seen more markedly in the spleen and liver of susceptible BALB nu/+ and nu/nu mice than in those of resistant CDF1. At a moribund stage, leukocyte infiltration was not present in the liver of BALB nu/+ and nu/nu mice, whereas mononuclear cells populated the lesions in the liver of CDF1 mice at the same stage. The number of circulating leukocyte was also significantly smaller in BALB nu/+ and nu/nu mice than in CDF1 mice at a moribund stage. These findings suggest that cell infiltration in the liver, major target organ for MHV growth, plays a role in determining the susceptibility to the virus.
View full abstract
-
Shotaro TAKEUCHI, Yoshinori KOBAYASHI, Tetsuo MOROZUMI
1985 Volume 47 Issue 5 Pages
769-775
Published: October 15, 1985
Released on J-STAGE: February 13, 2008
JOURNAL
FREE ACCESS
The purification of protease, produced by Staphylococcus hyicus subsp. hyicus strain No.111, was performed by successive precipitations with ammonium sulfate and cold acetone, chromatography on DEAE-cellulose and gel filtration on Sephadex G-75. By the procedure, the protease was purified 474.1-fold, and its recovery rate was 34.9%. The purity of the preparation was demonstrated by polyacrylamide gel electrophoresis, the immunodiffusion test and immunoelectrophoresis. The protease was most active at a neutral value of pH. The protease activity was inhibited by the presence of Cu, Zn, Ag, Fe, or Hg ions, but was slightly stimulated by Ca ion. No protease was stimulated by the reducing agents such as cysteine, 2-mercaptoethanol and sodium thioglycolate, and it was strongly inhibited by the addition of EDTA. The molecular weight of the purified protease was estimated to be 32, 000 by SDS-polyacrylamide gel electrophoresis.
View full abstract
-
Masaharu NAIKI, Ranjan RAMASAMY, James O. OCHANDA, Godfrey MAINA
1985 Volume 47 Issue 5 Pages
777-786
Published: October 15, 1985
Released on J-STAGE: February 13, 2008
JOURNAL
FREE ACCESS
Schistosoma mansoni adult worms obtained from human patients and mice infected with cercariae were compared in lipid composition. Both worms possessed cholesterol ester, free fatty acid and cholesterol as neutral lipids, but the latter worms possessed triacylglyceride as another component. Phospholipid compositions of both worms were similar. Larger amounts of phosphatidyl choline diacylglyceride and smaller amounts of phosphatidyl ethanolamine diacylglyceride were detected. Adult worms from infected mice were surface-labeled by galactose oxidase treatment followed by sodium boro[
3H]hydride reduction after or before trypsin treatment. Trypsin treatment was approximately 10 times more effective to label glycolipids than non-treatment. Mono-, di-, tri- and tetra-glycolyl components were labeled with trypsin treatment; however, mainly tri-glycolyl glycolipid was labeled without trypsin treatment. These results indicated that some glycolipid components are really exposed on the surface of the tegumental membrane. But no antigenicity was detected in the purified neutral and acidic glycolipid fractions by the Ouchterlony's double diffusion test using rabbit anti-S. mansoni sera or those obtained from patients with S. mansoni infection, though these sera, each gave two different precipitin lines against homogenate of the adult worms.
View full abstract
-
Masahiro TAGAWA, Akira TAKIYAMA, Hiroyasu EJIMA, Kazuo KUROKAWA
1985 Volume 47 Issue 5 Pages
787-790
Published: October 15, 1985
Released on J-STAGE: February 13, 2008
JOURNAL
FREE ACCESS
To examine the prophylactic efficacy of milbemycin D against Dirofilaria immitis infection, 59 filaria-free beagles were inoculated with 50 to 100 infective larvae of the parasite. Milbemycin D was administered orally at ranges from 0.05 to 3.2 mg/kg of body weight one month after inoculation or at the rate of 1 mg/kg of body weight one to 90 days after inoculation. All dogs were sacrificed for autopsy 6 months after inoculation to confirm the establishment of D.immtis infection. Complete protection from the infection was observed by treating with a dose of 1 mg/kg or more of milbemycin D one or two months after infection.
View full abstract
-
Masato UEHARA, Toshihiko UESHIMA
1985 Volume 47 Issue 5 Pages
791-798
Published: October 15, 1985
Released on J-STAGE: February 13, 2008
JOURNAL
FREE ACCESS
This study described the fine structures of glial cells, astrocytes, oligodendrocytes and microglial cells, in the ventral horn of the chicken spinal cord. The quantitative study revealed that astrotytes comprised about 49% of the total glial cells, oligodendrocytes 47% and microglial cells 4%. As compared with mammalian species such as rat, mouse and cat, the chicken spinal cord appeared to contain more astrocytes and fewer oligodendrocytes. Oligodendrocytes were divided into three subtypes, light, medium-dense and dark, according to the classification of Mori and Leblond [12]. Among these, medium-dense cells constituted about 80% of the total oligodendrocyte population.
View full abstract
-
Kanji YAMASAKI, Chitoshi ITAKURA
1985 Volume 47 Issue 5 Pages
799-802
Published: October 15, 1985
Released on J-STAGE: February 13, 2008
JOURNAL
FREE ACCESS
Osteosclerosis occurred in a high incidence in the sternum and tibia of 160 ICR mice examined histologically, at more than 300 days of age in the females and at more than 450 days of age in the males. It was initiated with a proliferation of osteogenous tissues at the metaphysis, resulting in replacement of the marrow cavity by a network of mature osseous trabeculae. There were osteochondrosis, osteoarthrosis and osteoporosis in mice affected with osteosclerosis. It was thought that osteosclerosis in the present mice might have occurred as one of the aging changes or a compensatory event for the weakened bone subsequent to the other bone changes.
View full abstract
-
Masao ASARI, Shin WAKUI, Kosaku FUKAYA, Yutaka KANO
1985 Volume 47 Issue 5 Pages
803-806
Published: October 15, 1985
Released on J-STAGE: February 13, 2008
JOURNAL
FREE ACCESS
Formation of the bovine spiral colon was studied in fetuses between 2.1 cm and 25.0 cm crown-rump length. The first evidence of looping of the proximal colon was seen at 3.5 cm. By 6.5 cm the looping colon was beginning to assume the form of a coil, and by 9.3 cm the coiling colon was beginning to appear conical. The conical proximal colon athieved the elliptical disc-like adult form with centripetal and centrifugal coils between the 13 cm to 23 cm stage.
View full abstract
-
Shigeyoshi ITOHARA, Itsuro OIKAWA, Shinichi TERUI, Yoshio MIZUNO
1985 Volume 47 Issue 5 Pages
807-810
Published: October 15, 1985
Released on J-STAGE: February 13, 2008
JOURNAL
FREE ACCESS
Infectivities of bovine leukemia virus (BLV) in peripheral blood lymphocytes (PBL) from naturally infected cattle were examined by the syncytium infectivity assay. Infectious BLV was detected in PBL from all cattle which were positive in the immunodiffusion test using glycoprotein antigens of BLV. The infectious titers per 5 × 10
5 PBL from individual cattle were closely associated with the severity of persistent lymphocytosis, but not with the antibody titers to both the glycoprotein and internal protein antigens of BLV.
View full abstract
-
Aisuke NII, Hiroyuki NAKAYAMA, Reiji TAKAHASHI, Kosaku FUJIWARA
1985 Volume 47 Issue 5 Pages
811-815
Published: October 15, 1985
Released on J-STAGE: February 13, 2008
JOURNAL
FREE ACCESS
Ten cases of canine primary lung tumor were studied pathologically, revealing anaplastic carcinoma (3 cases), squamous cell carcinoma (3 cases), adenosquamous carcinoma (2 cases), and adenocarcinoma (2 cases).
View full abstract
-
Kanji MATSUI
1985 Volume 47 Issue 5 Pages
817-821
Published: October 15, 1985
Released on J-STAGE: February 13, 2008
JOURNAL
FREE ACCESS
Fetal electrocardiogram in a twin pregnancy of a Thoroughbred horse was recorded. Two colts were born alive at 340 days of gestation. One was healthy but the other was growth-retarded. Both fetal heart rates decreased similarly with gestational age, but the discrepancy was noted from 4 days before parturition. The higher heart rate seemed to be obtained from the growth-retarded colt. Maternal heart rate increased gradually with the advancement of gestational period and then increased prominently during the last 2 weeks before parturition.
View full abstract
-
Hideo MURATA, Hideyuki TAKAHASHI, Hideto MATSUMOTO
1985 Volume 47 Issue 5 Pages
823-827
Published: October 15, 1985
Released on J-STAGE: February 13, 2008
JOURNAL
FREE ACCESS
Effects of 1 hour transportation by a truck on cellular immune function were investigated in Holstein castrated calves of 6 months of age. Mitogen-induced blastogenesis of lymphocytes and Nitroblue tetrazolium (NBT) reduction activity of phagocytes in peripheral blood were determined before and 6 and 24 hours after the transportation and compared with those of control animals. Increases were observed in both of the activities 6 hours after the transportation. The increments returned to the initial level within 24 hours. These results suggest a temporary enhancement of cellular immune function due to the transportation.
View full abstract
-
Katsumi KUME, Toyotsugu NAKAI
1985 Volume 47 Issue 5 Pages
829-833
Published: October 15, 1985
Released on J-STAGE: February 13, 2008
JOURNAL
FREE ACCESS
A heat-labile dermonecrotic toxin was purified from sonic bacterial extracts of serotype D strain of Pasteurella multocida of pig origin. The purified toxin was a protein with a molecular weight ca. 160, 000. Treatment of the mildly trypsinized toxin with dithiothreitol and sodium dodecyl sulfate (SDS) produced three polypeptide chains of molecular weight of ca. 23, 000 (fragment a), ca. 67, 000 (fragment b), and ca. 74, 000 (fragment c). Without pretreatment with trypsin, dithiothreitol and SDS failed to dissociate the polypeptides. None of the dissociated fragments showed detectable toxicity.
View full abstract
-
Masami YASUDA, Takeshi MIKAMI, Hisao IZAWA, Kazuyoshi IKUTA, Shiro KAT ...
1985 Volume 47 Issue 5 Pages
835-839
Published: October 15, 1985
Released on J-STAGE: February 13, 2008
JOURNAL
FREE ACCESS
Three monoclonal antibodies (G4, B2 and C12) that were positive for the virus intracellular antigens of cells infected with herpesvirus of turkeys (HVT) in the immunofluorescence assay were established. These antibodies did not react with the membrane antigen of cells infected with HVT or Marek's disease virus and were negative for its homologous virus in the agar gel precipitation and neutralization tests. C12 antibody stained irregular granules in cytoplasm, and G4 and B2 antibodies showed diffuse cytoplasmic staining. By immunoprecipitation and analysis of SDS-polyacrylamide gel electrophoresis G4 and B2 antibodies precipitated the 30 kilodaltons (K), 24K and 20K polypeptides and C12 antibody slightly precipitated the 70K and 45K polypeptides in HVT-infected cells.
View full abstract
-
Shotaro TAKEUCHI, Yoshinori KOBAYASHI, Tetsuo MOROZUMI, Seiichi NIIBOR ...
1985 Volume 47 Issue 5 Pages
841-843
Published: October 15, 1985
Released on J-STAGE: February 13, 2008
JOURNAL
FREE ACCESS
Staphylococcus hyicus subsp. hyicus was isolated from 97 (23.7%) out of 408 healthy pigs, from 55 (18.9%) out of 290 healthy chickens and from 6 (6.2%) out of 96 bovine udders, respectively. All of the isolates (n=204) were positive in Tween 80 hydrolysis, heat-stable DNase and hyaluronidase, but negative in·hemolysin, clumping factor and coagulase. In particular, most of them showed strong reactions in gelatin hydrolysis and in the fibrinolysin test. In these properties, there was no difference as to the origin of the isolates.
View full abstract
-
Kenji MACHII, Yuko OTSUKA, Hiroshi IWAI, Katsumoto UEDA, Eiko SUZUKI, ...
1985 Volume 47 Issue 5 Pages
845-848
Published: October 15, 1985
Released on J-STAGE: February 13, 2008
JOURNAL
FREE ACCESS
Enzyme-linked immunosorbent assay (ELISA) was applied to diagnose Mycoplasma pulmonis (Mp) infection in rats. The assay was more sensitive than complement fixation test and had about the same sensitivity as isolation of Mp from the respiratory tract except very early stage (1 week) of the infection. Since Mp shared cross-reactive antigen with M. arthritidis (Ma), parallel use of both Mp and Ma antigens was recommended for diagnosis of Mp infection when sonicated antigens were used.
View full abstract
-
Masaaki FUKUDA, Toyoaki HAYAMA
1985 Volume 47 Issue 5 Pages
849-850
Published: October 15, 1985
Released on J-STAGE: February 13, 2008
JOURNAL
FREE ACCESS
Effects of intravenously infused adenosine on the ruminal electromyogram (EMG) and heart rate were investigated in conscious goats. Pompt inhibition of the ruminal EMG and tachycardia were observed by a low dose (0.2-0.5 mg/min) of adenosine. The responses were rapidly disappeared by quit of infusion. The positive chronotropic action seems to be species specific in goat.
View full abstract
-
Yoichi OGHISO, Yoshihisa KUBOTA, Satoshi FUKUDA, Haruzo IIDA
1985 Volume 47 Issue 5 Pages
851-854
Published: October 15, 1985
Released on J-STAGE: February 13, 2008
JOURNAL
FREE ACCESS
Alveolar macrophages (AM) from the beagle can release interleukin 1 (IL-1) by the exposures to dust particles. IL-1 activity was significantly detected after the exposures to silica and asbestos particles in vitro, and this induction was enhanced in the presence of LPS. This result indicates that AM may have an immunostimulatory effect in the lungs after the inhalation exposures to dust particles.
View full abstract
-
Hideyuki TAKAHASHI, Hideo MURATA, Hideto MATSUMOTO
1985 Volume 47 Issue 5 Pages
855-858
Published: October 15, 1985
Released on J-STAGE: February 13, 2008
JOURNAL
FREE ACCESS
The effect of the heat exposure on the plasma levels of insulin, glucagon and 11-hydroxycorti-costeroids (11-OHCS) in calves was studied by employing an arginine tolerance test. Those hormone levels increased transiently following an arginine injection. The response in plasma insulin due to arginine was inhibited by the heat exposure. Meanwhile, glucagon and 11-OHCS responses due to arginine were enhanced by the heat exposure.
View full abstract
