Abstract
Three monoclonal antibodies (G4, B2 and C12) that were positive for the virus intracellular antigens of cells infected with herpesvirus of turkeys (HVT) in the immunofluorescence assay were established. These antibodies did not react with the membrane antigen of cells infected with HVT or Marek's disease virus and were negative for its homologous virus in the agar gel precipitation and neutralization tests. C12 antibody stained irregular granules in cytoplasm, and G4 and B2 antibodies showed diffuse cytoplasmic staining. By immunoprecipitation and analysis of SDS-polyacrylamide gel electrophoresis G4 and B2 antibodies precipitated the 30 kilodaltons (K), 24K and 20K polypeptides and C12 antibody slightly precipitated the 70K and 45K polypeptides in HVT-infected cells.
