Application of Enzyme-Linked Immunosorbent Assay (ELISA) to Detection of Antibodies against Tyzzer's Organism (Bacillus piliformis) in Mice

Abstract

Enzyme-linked immunosorbent assay (ELISA) was developed for detecting antibody against Tyzzer's organism "Bacillus piliformis" in immune mouse sera, using formalin-killed organisms grown on a primary mouse hepatocyte culture. There was good correlation on titers between ELISA and immunofluorescence, while ELISA titers were lower. ELISA was shown to be useful for studying antigenic relatedness among different bacterial strains.