Isolation of Transmissible Gastroenteritis Virus from Feces of Diarrheic Pigs in Roller Culture of CPK Cells in the Presence of Trypsin

Abstract

A new techinique was established for the isolation of TGE virus from the feces of diarrheic pigs. Cytopathic TGE viruses were isolated consistently in roller cultures of CPK cells by a combination of trypsin treatment of the inoculum and incorporation of trypsin in the maintenance medium.