Abstract
The antibody to ganglioside GD3, which was invariably increased in tumor lymph node cells of enzootic bovine lymphosarcoma (EBL), was examined for its possible usefulness in detecting EBL tumor cells. The anti-GD3 antibody was prepared by immunization of chicken with GD3 from EBL tumor lymph node cells. The antibody to ganglioside GD3 reacted to ganglioside GD3 containing N-glycolylneuraminic acid from EBL tumor cells. The reactivities of anti-GD3 antibody to peripheral blood lymphocytes (PBL) from aleukemic and leukemic cattle and tissues of bovine fetuses were examined by complement-dependent antibody cytotoxicity test. The antibody to ganglioside GD3 had cytotoxic activity to PBL from EBL cattle, fetal bovine thymus and liver cells but not to PBL from healthy cattle irrespective of presence or absence of bovine leukemia virus infection. In addition, acctone-fixed EBL tumor cells showed a positive reaction to the antibody to ganglioside GD3 in an indirect fluorescent antibody test.
