Abstract
We previously reported that Marek's disease (MD) lymphoma-derived cells, transplantable MD tumor-derived cells and lymphoid leukosis (LL) lymphoma-derived cells, each express different glycosphingolipid (GSL) antigens. The GSL composition of each cell line was compared by thin-layer chromatography and immunological analysis. Six different cell lines derived from MD lymphoma showed a typically identical GSL composition, in which a unique neutral GSL with a carbohydrate chain consisting of more than five sugars was detected as a major component. However, transplantable MD tumor-derived cell lines and LL lymphoma cell lines showed a similar composition in which Forssman GSL, asialo GM1 ganglioside and other several components were detected. The specific presence of Forssman GSL and asialo GM1 in these two cell lines was confirmed by immunological reaction of GSLs from each cell line with antisera to each authentic GSL and by the specific antibody elevation in rabbits immunized with each cell line.
