Abstract
The effects of canine serum on the cellular configuration in vitro were studied in polymorphonuclear leukocytes (PMN) from the peripheral blood of clinically healthy adult beagle dogs. PMN rapidly changed their shape from spherical to ruffled, uropod, or polarized within 1 to 5 min after incubation with freshly isolated autologous or allogeneic canine serum at 37°C, as observed in PMN incubated with zymosan-activated serum and casein. The maximal shape change response (approximately 68%) was observed at 5 min after PMN were exposed to 10% serum. The neutrophils changed their shape faster and at a higher percentage than did eosinophils. Furthermore, the heat treatment (56°C for 30 min) of serum did not affect the PMN shape change response. These results indicate that serum has a potent effect on the induction of the PMN shape change response.
