Preparation of Antibodies Recognizing the New Group Antigens of Feline Red Blood Cell

Abstract

Anti-C1 and Anti-C2 antibodies were obtained successfully by immunization of feline red cells to rabbits. The activities of anti-C1 and anti-C2 were seen in the IgM and the IgG fractions, respectively. With anti-C1, red blood cell groups were divided into 2 groups, C1 (+) type and C1 (-) type. With anti-C2, red cell types likewise were classified into 2 groups, C2 (+) type and C2 (-) type. By the absorption of anti-C2 with various C2 (+) type red cells, it was distinguished into 4 parts, anti-C2abcd, -C2bcd, -C2cd, and -C2d. The C2 (+) type were recognized in 4 parts, C2a type, C2ab type, C2abc type, and C2abcd type, and these C2 (+) antigens were assumed to be dosage characters. The inheritance mode of C1 (+) type and C2 (+) type was not clearly determined. From the results of comparison test between anti-C1, -C2 and naturally occurring antibodies (anti-Ca, -Cb), the former was corroborated to be new antibodies.