Abstract
Porcine parvovirus formed clear plaques on 3- and 24-hr-culture monolayer of embryonic swlie kidney cell line, ESK cells, by seeding with 3 ml of cell suspension containing 4×105 cells per ml in each well of the Costar's plate. Infective titer by plaque assay on 3-hr-culture monolayer was higher than that on 24-hr-culture monolayer. Plaques on 48- and 72-hr-culture monolayer were dull and uncountable. Plaques became large but infective titer did not increase when diethylaminoethyl-dextran was incorporated in the overlay medium. Plaque formation was specifically inhibited by antiserum against the virus. The virus grew well in 3-hr-culture monolayer and eclipse phase of the virus growth was between 8 and 10 hr.
