Abstract
Bovine (α1-acid glycoprotein (α1AG) was purified from pooled normal bovine sera by successive ammonium sulfate precipitation, ion-exchange chromatographies and gel filtration. Bovine α1AG had a molecular weight of 42, 000±2, 000 and a sedimentation coefficient of 3.4S. It contained 26.6% carbohydrate. Gel isoelectric focusing revealed a microheterogeneity with 7 to 8 bands in a pI range of 3.2 to 3.7. It migrated to the α1-globulin region upon immunoelectrophoresis. Single radial immunodiffusion was developed for the quantitative measurement of bovine α1AG in serum. The mean serum value of α1AG in 152 healthy Holstein cattle (1-12 years old) was 283.2±82.3pg/ml. Elevated values (cut-off value=450pg/ml) were observed in cattle with traumatic pericarditis (100%), arthritis (100%), mastitis (91%), pneumonia (70%), and mesenteric liponecrosis (43%).
