Modification of polystyrene surface by negative ion implantation for improving and patterning attachment properties of neurons was investigated. This modification technique is required for artificial formation of neural network with neuron cells and for development of “bio-interface” between vital neural system and external silicon circuit. Silver-negative ions were implanted into various kinds of polystyrene surfaces through a mask with slit array of 60μm in width and 4 mm in length with 60μm spacing. In the cell culture of PC-12h (rat adrenal pheochromocytoma) cells with the ion-implanted polystyrenes, PC-12h cells attached and neurites extended only on the ion-implanted regions of untreated polystyrene dishes (NTPS) and spin-coated polystyrene (SCPS). This means that pattering ion implantation is able to make patterning treatment both of cell attachment and neurites extension properties.
