Development of nano-endoscopy for visualizing inside of living cells

Abstract

In this study, we have been developing nano-endoscopy based on 3D-AFM technique. We established a tip fabrication process for inserting the tip into the cell with keeping cells alive, and visualized 3D structures inside the cell in real space. By the developed method, we open the possibility for visualizing intracellular nano-meter scale dynamics which cannot be seen with a super-resolution microscopy.