Host: The Japan Society of Vacuum and Surface Science
We prepared cytochrome c oxidase (CcO)-modified gold electrodes through self-assembled monolayers (SAM) of mixed alkanethiols and tracked the surface assembly of bilayer lipid membranes (BLM) and the binding of cytochrome c (Cyt c) to the electrode by time-resolved surface-enhanced infrared absorption (SEIRA) spectroscopy. The SEIRA spectra suggest that terminal functional groups of alkylthiols used for the SAM affected the CcO orientation at the surface and binding amounts of Cyt c to the surface-tethered CcO. Electrochemical measurements of CcO-modified electrodes were performed before and after the Cyt c binding in an oxygen atmosphere. Catalytic cathodic currents, which are associated with the oxygen reduction catalyzed by the CcO, were observed and were increased after the binding of Cyt c.
