2018 Volume 61 Issue 1 Pages 11-20
The diagnosing Mycobacterium avium（M. avium） that causes swine mycobacteriosis takes a lot of time, because M. avium grows slowly on a medium traditionally used. In order to develop a more-speedy and simple method for detection of swine mycobacteriosis from specimens collected from in pig farms, we examined the MGIT medium for culturing and isolating M. avium following pre-culturing treatments （MGIT method）. Moreover, we compared a PCR （IS901） method with a PCR-RFLP （hsp65） method as an identifying method for M. avium from wild-type Mycobacteria strains isolated from pig farms.
The detection limit of the MGIT method was about ≧ 2×102 CFU per 1g of litter from pig farms, the period required for a positive judgment was ≦ 10 days after starting the culture.
Of 38 wild-type Mycobacteria strains, identification results relating to Mycobacterium avium complex from the both methods matched for 33 strains. The remaining 5 strains were determined to be M. avium by the PCR（IS901） method, while they were unidentified by the PCR-RFLP（hsp65） method.
In conclusion, the MGIT method and the PCR（IS901） method can be useful as methods for isolating and identifying M. avium from environments of pig farms.
Nihon Chikusan Gakkai Kyushu Shibuho
Nihon Chikusan Gakkai Nishinihon Shibuho
