Experimental Studies on the Candida Albicans Successive infection tests by

means of simultaneous inocuration of Candida albicans, antibiotics, VB₁ and glucuronic acid into mice

Abstract

Candida albicans alone or one of the following drugs-antibiotics (aureomycin, penicillin), VB1, glucuronic acid-together with Candida albicans was inoculated into the abdominal cavity and successive infection tests were carried out until the 10th generation, in order to compare the pathological and biological changes of the organs and the Candida respectively caused by each of the drugs. The results were as follows:
1) The degree of pathological changes increased with the generation. Tetracycline derivates (aureomycin) showed the most remarkable changes and had an especially great infiuence upon the Candida infection.
In the mice inoculated with both VB₁ and penicillin, the degree of pathological changes was lower than in those inoculated with candida alone.
2) However, remarkable cultural results were frequently obtained from internal organs which demonstrated no marked macroscopical changes.
3) In the maltose fermentation test, the strain of the 10 th generation isolated from the mice inoculated with both Candida albicans and VB₁ did not produce gas, though the contrast strains isolated from the mice inoculated with Candida alone or with the other strains did, and the former was inferior to the other strains in the degree of acid production.
In the sucrose fermentation test, this strain of the 10 th generation was negative in the acid production, whereas the other strains of the same generation demonstrated a little higher degree of acid production, as compared with the contrast one and each of the corresponding ones of the 5 th generation.