Studies on the Inoculation of Brucella into Embryonated Hen Eggs

Abstract

Based on the bacteriological and serological findings following the inoculation of Brucella abortus Bang (B 3 Bang) into embryonated hen eggs, a therapeutic experiment was undertaken and the results obtained were as follows.
It was found that the embryonated egg was highly susceptible to Brucella, and relatively good growth of the microorganisms was gained in the yolk and on the chorioallantoic membrane. The sensibility of the egg to Brucella was demonstrated to be high with decreasing age of the embryonated egg. Such an infection began in all parts of the egg gradually from about 48 to 72 hours after the inoculation and the systemic infection developed rapidly in the embryo, the phenomenon of the infection resembling the aspect of the human natural disease.
An attempt was made to evaluate therapeutic effects following administration of sera or antibiotics (Penicillin, Streptomycin, Aureomycin, Terramycin, Achromycin, Chloramphenicol, Oleandomycin and Thiasin) into the embryonated egg inoculated with Brucella. The results indicated that the therapeutic effect was the most significant in Streptomycin, good in Penicillin and Tetracyclines such as Terramycin, Acromycin, etc., very slight in Oleandomycin, and the most poor in Chloramphenicol, slight response having been found in the administration of sera.
These findings mentioned above seem to give a new suggestion for the treatment of brucellosis, although the findings in vitro and in vivo are slightly different.
The embryonated eggs demonstrated no productivity of the antibody to the Brucella strain. No biological and serological changes in this strain were demonstrated following continuous culture by passage of the Brucella into the yolk sac, although the susceptibility of mice to the Brucella decreased following the continuous culture. The S-R tra nsformation was not found following the continuous passage.