Candida albicans alone or one of the following drugs-antibiotics (aureomycin, penicillin), VB1, glucuronic acid-together with Candida albicans was inoculated into the abdominal cavity and successive infection tests were carried out until the 10th generation, in order to compare the pathological and biological changes of the organs and the Candida respectively caused by each of the drugs. The results were as follows:
1) The degree of pathological changes increased with the generation. Tetracycline derivates (aureomycin) showed the most remarkable changes and had an especially great infiuence upon the Candida infection.
In the mice inoculated with both VB₁ and penicillin, the degree of pathological changes was lower than in those inoculated with candida alone.
2) However, remarkable cultural results were frequently obtained from internal organs which demonstrated no marked macroscopical changes.
3) In the maltose fermentation test, the strain of the 10 th generation isolated from the mice inoculated with both Candida albicans and VB₁ did not produce gas, though the contrast strains isolated from the mice inoculated with Candida alone or with the other strains did, and the former was inferior to the other strains in the degree of acid production.
In the sucrose fermentation test, this strain of the 10 th generation was negative in the acid production, whereas the other strains of the same generation demonstrated a little higher degree of acid production, as compared with the contrast one and each of the corresponding ones of the 5 th generation.

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Based on the bacteriological and serological findings following the inoculation of Brucella abortus Bang (B 3 Bang) into embryonated hen eggs, a therapeutic experiment was undertaken and the results obtained were as follows.
It was found that the embryonated egg was highly susceptible to Brucella, and relatively good growth of the microorganisms was gained in the yolk and on the chorioallantoic membrane. The sensibility of the egg to Brucella was demonstrated to be high with decreasing age of the embryonated egg. Such an infection began in all parts of the egg gradually from about 48 to 72 hours after the inoculation and the systemic infection developed rapidly in the embryo, the phenomenon of the infection resembling the aspect of the human natural disease.
An attempt was made to evaluate therapeutic effects following administration of sera or antibiotics (Penicillin, Streptomycin, Aureomycin, Terramycin, Achromycin, Chloramphenicol, Oleandomycin and Thiasin) into the embryonated egg inoculated with Brucella. The results indicated that the therapeutic effect was the most significant in Streptomycin, good in Penicillin and Tetracyclines such as Terramycin, Acromycin, etc., very slight in Oleandomycin, and the most poor in Chloramphenicol, slight response having been found in the administration of sera.
These findings mentioned above seem to give a new suggestion for the treatment of brucellosis, although the findings in vitro and in vivo are slightly different.
The embryonated eggs demonstrated no productivity of the antibody to the Brucella strain. No biological and serological changes in this strain were demonstrated following continuous culture by passage of the Brucella into the yolk sac, although the susceptibility of mice to the Brucella decreased following the continuous culture. The S-R tra nsformation was not found following the continuous passage.

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A mass incidence of food poisoning occured in a seaside school opened at Asamushi Hotspring of Aomori Prefecture among pupils who came from Morioka City, Iwate Prefecture. After their returning to Morioka, the feces of 31 patients were examined bacteriologically, and gram-negative rods demonstrating general characteristics of Salmonella were isolated from 20 of the patients. The organisms with an antigenic structure 9: 12, gm, proved to be serologically entirely identical with Salmonella enteritidis by the cross-agglutination test. Further, all the isolated strains were put to examination for resistance test to various antibiotics and sulfonamide giving the following, results: the strains were resistant to penicillin at 10 uicc; to both chlortetracycline and oxyteracycline at 60 γ/cc; and to sulfaisoxasol at 250 γ/cc.
It was noted that 10^-7 mg/cc suspension of the organisms killed mice of about 20 mg weight within a week after its intraperitoneal inoculation.
Feeding experiments with the mice of the biscuit which were carried back from Asamushi by some of the patients only gave negative results
Thus, the source of infection could not be made clear. The convalescent serum showed rise in the value of antibody to both the standard strain and the isolated strain of Salmonella, enteritidis Conclusively, because the 20 strains were all isolated using non-selective medium, it was keenly felt that non-selective medium should be used together with selective medium in the isolation of pathogenic Enterobacteriacea performance of sucha study.

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The Schick and Moloney tests were given to 146 older children and 55 adults and the antitoxin titer in their sera were measured, obtaining the results as follows:
1) Almost all the cases who showed a negative Schick reaction, revealed over 1/30 AU/ml (Schick level) and none of them below 1/1000 AU/ml.
2) Relatively large number of cases showed to have sufficient antitoxin titer even among those who showed a positive Schick reaction.
3) Many cases were able to obtain an increased antitoxin titer, after having received the Moloney test which was previously thought only to show sensitivity to toxoid. Especially 82.6% of the cases, which had the titer ranging between 1/1000 to 1/30 AU/ml, demonstrated a high increase of the titer over 1/30 AU/ml, except for one case. The cases which showed a strong positive Moloney reaction, even with antitoxin titer below 1/1000 AU/ml in their serum, were recognized to show a satisfactory increase of the titer after the Moloney test.
According to the results of Schick and Moloney tests, it is possible to choose adequate persons who need to have the passive immunization, the booster immunization, of diphtheria toxoid. That is:
1) The persons having a negative Schick reaction need no booster injection.
2) The persons having a positive Schick reaction should be divided into three groups depending upon the severity of the Moloney reaction.
a) The strongly positive group (++) does not need to have the toxoid.
b) Among the moderately positive group (+), only those who are still positive to the Schick test after one month, should receive a minute dosage of the toxoid.
c) The negative group (-) should receive the toxoid in full dosage immediately.
It will be concluded that the past objectable side effect is able to be prevented and the more safe and reliable effect of prevention is expected by tihs method.

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A species of halophilic bacteria was cultivated from the stool of patients affected by food poisoning in 1955 at Yokohama National Hospital, and it was acknowledged to be the causative agent of the disease.
It was proposed to name the bacteria Pseudomonrs enteritis, and Pasteurella parahemolitica Fujino 1950 has the same properties.
Since 1958 at many laboratories in Miyagi (Sendai), Tokushima, Kobe, Kanagawa (Yokohama) and Miyazaki (Hyuga) numerous cases of food poisoning, epidemic or sporadic, proved to be the infection of this species of bacteria. (Table 7 and Fig. 2)
The biological nature of Pseudomonas enteritis is shown in tables 2 and 3. The bacteria is regarded as of marine source.
Pseudomonas enteritis has two kinds of antigen, thermo-labil and thermo-stable, and a large number of the strains were classified into twenty antigen types (Table 7 and 3)
Cardinal symptoms of the patients infected by Pseudomonas enteritis are shown in table 1 and figure 1, that is to say, abdominal pain and diarrhea are common.
Causative foods are shown in table 4, and they are generally more or less connected with marine products.
In the cases in Tokushima the bacteria with the same natures were detected from both patient's materials and suspected foods, by applicating the immun sera of the bacteria for agglutination test.
The occurrence of the disease is limitted only in summer, from June to October, most frequently in August, and only one sporadic case in the beginning of November. (Fig. 3)
Number of patients in each age interval is shown in table 6.
Extremely rapid and abundant growth of this species of bacteria must be significant for pathogenity.
Fly may have some reference to the infection as a carrier.
Vibrio cholerae has a similar nature of halophile as shown in figure 4. It is very interesting that they have common natures in many respects. This information may offer some new views on the epidemiology and examination method of cholerae.

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