Examinations of pathogenic Escherichia coli, Staphylococcus and Pseudomonas from various objects, new-born infants and workers in the neonatal intensive care unit in the college hospital were performed in 1973 and 1979. In 1978 new nursery which equipped the clean air ventilation and pre-room for incoming person was opened in the hospital ward newly constructed and had been used for 8 months until this study.
The results obtained as follows:
1. Pathogenic Excherichia coli was not isolated from any specimen.
2. Staphylococcus aureus was isolated from sink and catheters for nutrition. The isolates showed resistance to penicillin, erythromycin, and sulfoisoxasol. Staphylococcus epidermidis was isolated from fingers of all of doctors and nurses working in nursery.
3. Pseudomonas aeruginosa was isolated from sink, catheters for nutrition and thermometer and humidifier of incubators in both examinations, and also from the throats of new-born infants in 1973 and from the throats of new-born infants and nurses in 1979. The isolates showed sensitivity to gentamicin.
4. The results suggest that new-born infants and medical care takers are capable both of recieving and of mediating microbial contaminations, and also new-born infants, nurses and spots retentive of moisture are easily contaminated by Pseudomonas aeruginosa.
