Abstract
The antitumor effect of human interferon-γ (IFN-γ) with or without polyprenoic acid (E5166) on human hepatoma cells (HuH-7) transplanted into nude mice was investigated. Nude mice xenografted tumors were divided into 8 groups and treated for 2 weeks in accordance with different dose of IFN-γ (2×103IU, 1×104IU, 5×104IU, 1×105IU) with or without E5166 (40mg/kg). In the groups treated with IFN-γ alone, a dose of 1×105IU/day showed the most remarkable antiproliferative effect to transplanted HuH-7 cells. However, this effective with IFN-γ was not always dose dependent. On the other hand, in the groups treated with combination of IFN-γ and E5166, additional antitumor effect of E5166 to IFN-γ was not observed, although a combination of IFN-γ in dose of 5×104IU/day and E5166 showed the same effect as IFN-γ in a dose of 1×105IU/day. In conclusion, it is suggested that administration of IFN-γ in higher dose is useful in therapy of hepatocellular carcinoma and combination with IFN-γ and E5166 can reduce the dose of IFN-γ.
