その他
一次性拘縮肩の関節滑膜における遺伝子発現解析
2011 年 35 巻 2 号 p. 613-616
詳細
2011 年 35 巻 2 号 p. 613-616
Primary frozen shoulder is a condition characterized by painful and limited active and passive range of motion, and its etiology is still uncertain. In the present study, we quantified the relative gene expressions in the synovium of glenohumeral joints between five primary frozen shoulders and seven non-frozen shoulders by using real-time reverse transcriptase-polymerase chain reaction (RT-PCR) in order to clarify the pathogenesis of frozen shoulders. Synovium samples were obtained from an anterior rotator interval area in arthroscopic surgery. Gene expressions of type I and type III procollagen and CD34 antigen in the joint synovium of frozen shoulders were significantly greater than those in the synovium of non-frozen shoulders. All genes of three hyaluronan synthase isoforms were expressed in the synovium of non-frozen shoulders, however, the gene expressions of hyaluronan synthase were less or lost in the synovium of frozen shoulders. The genes of interleukin (IL) -1α, IL-1β, and tumor necrosis factor (TNF)-α, which were expressed in the initial phase of inflammation, were expressed at low level or not expressed at all in the synovium of both frozen and non-frozen shoulders. The gene of IL-8, which induces angiogenesis, was predominantly expressed in frozen shoulders and the gene of IL-10, which is anti-fibrosis, was predominantly expressed in non-frozen shoulders. The gene expressions of interferon-γ, which induces fibrosis and adhesion, in the synovium of frozen shoulders were significantly greater than those in the synovium of non-frozen shoulders. These findings indicated that the fibrosis and adhesion in the synovium of frozen shoulders were induced with high-expressed interferon-γ and IL-8 and that the production and secretion of high molecular weight hyaluronan, which is essential for the joint function and maintenance, were reduced in the synovium of frozen shoulders. Low production of high molecular weight hyaluronan may correlate with the pathogenesis of primary frozen shoulders.
