抄録
Background: Low-intensity pulsed ultrasound (LIPUS) has been used for fracture healing and its effectiveness has been confirmed. To prevent re-tear of the repaired rotator cuff, it is important to regenerate the tendon-to-bone interface after rotator cuff repair. Therefore, we have investigated the effect of LIPUS on human rotator cuff derived cells hypothesizing that their osteogenic activity would increase by LIPUS exposure.
Methods: Human rotator cuff derived cells were adapted to a six well plate. The medium was replaced with the osteogenic medium, and the cells were divided into the two groups. In the LIPUS group, LIPUS was applied for 20 minutes every day for 10 days. Cells without LIPUS exposure acted as a control group. Then they were analyzed by cell number count, Alizarin Red staining and real time PCR analyses.
Results: There was no significant difference in the total number of cells between the two groups at 10 days. Alizarin Red staining of LIPUS-treated cells was better stained than that of the control group after 10 days. In the LIPUS group, the expressions of ALP and Runx2 were significantly higher than those in the control group.
Discussion: This study showed that the osteogenic activity of human rotator cuff derived cells was increased by LIPUS exposure. These results could show a way for rotator cuff repair in early healing of the tendon-to-bone interface by LIPUS exposure.
