乳歯生活歯髄切断後の治癒機転に関する病理組織学的研究

抄録

After giving vital pulpotomy to deciduous teeth, by using Calvital and Dycal from calcium hydroxide, PV from formocresol, and FR-condensed compound of guaiacol and formaldehyde, the auther observed the process of recovering histopathologically, and concluded as follows : 1. In the amputated part of the pulp a necrosis layer is formed in using Calvital, FR and PV, but when Dycal is applied we can find little formation of it and sometimes not any of it. 2. Three days after the operation a thin calcificated tissue is formed on the boundary of the necrosis layer and the vital pulp, and it turns light yellowish red by van Gieson staining and dark purple by silver impregnation staining, and it is recognized as collagen stoma. Close to it we can observe spindle cells and round cells in the pulp clearly. In case of using Dycal and FR, the part becomes glassy and calcium is deposited there. In case of using PV, between three days and fourteen days fiber constituent increases. 3. After seven days have passed, in case of using Calvital, dentin barrier begins to be formed, and we can see big spindle cells - pre-odontoblast - there. 4. Between 21 days and 35 days, in case of using Calvital, predentin is found in the dentin barrier, and then odontoblast connects with odontoblast of root canal. In case of van Gieson staining method the dentin barrier turns dark yellowish red-purple, and dark red-purple by silver impregnation staining, and the fibers in the pulp and the dentin barrier are gitterfaser. Calcificated tissue remains a little roughly fibrous. In case of using Dycal, in about 21 days it is calcificated, and between 35 days and 62 days the dentin barrier is formed. In case of FR, the dentin barrier is formed and spindle cells, round cells and multiple cells are observed. At this stage it has the same process as the case of Calvital. In case of using PV, inflamatory cells, which chiefly consist of neutrocyte are observed in the pulp in the middle part of the root canal. 5. In case of using Calvital, FR and Dycal, between 62 days and 92 days the dentin barrier becomes broad and thick and turns red with acid fuchsin by van Gieson staining, purple by silver impregnation staining evenly as primary dentin. On the surface of the pulp rough fibrous tissue remains, and in the barrier a rather thick gitterfaser lies screwing towards predentin through odontoblast. In case of using PV, inflamatory cells continues to infiltlate and it sometimes develops to suppurative inflamation. 6. In case of using Calvital, FR and Dycal internal absorption is not observed, but in using PV we can find it in 35 days.