局所自家骨小片移植に関する病理組織学的研究

抄録

The author performed enucleation of the tooth germ in the 4th premolar of mandible of young adult dogs and grafted local autogenous bone chips. Observations by tetracycline labelling and microradiogram were made of the changes in the grafted bones and the recipient bones. Pathohistological investigations were also made of the non-decalcified and decalcified stained specimens. The results were as follows : 1. In the decalcified stained specimens, the formation of trabeculae from the wall of the defect into the cavity was observed on the 5th day after grafting, and the defect was filled with the new bone to the 30th day. These findings were the same as the non-decalcified findings in the tetracycline labelling and the microradiogram. 2. A slight bone neogenesis was seen taking place in the peripheries of the transplanted bone chips on the 5th day after grafting. On the 10th day, tetracycline labelled layer was found in the peripheries of the little transplanted bone chips. In microradiographic findings, it was recognized to be radiopaque. The new bone in the peripheries of the transplanted bone chips was connected with the new bone from the wall of the defect on the 15th day and the defect space was filled with the retiform new bone. 3. The nuclei of the osseous cells of the transplanted bone chips began to atrophy and disappear on the 5th day, and the osseous cells decreased and disappeared gradually by the 20th day. On the 30th day, the small cavities of the transplanted bone chips were found to be vacant. 4. The fibrous connective tissues and capillaries invaded into the Haversian canal of the transplanted bone chips on about the 15th day. On the 20th day, a new formed trabeculae was formed in the Haversian canal. And the formation of the new bone started from inside the transplanted bone chips. 5. Repair of the newly formed bone began on about the 30th day, and the osseous cells were arranged regularly, and the width and calcification of the trabeculae increased. This repair continued to the 90th day. 6. In the present experiment, the transplanted bone chips did not show the capacity for osteogenesis, but had the capacity to stimulate the peripheral soft tissues to regenerate bone. And they seem to have some part in osteogenesis and in bone union with the recipient bone. The repair of the transplanted bone chips was mainly a latent displacement.