成長期の虚弱骨に関する X 線的, 光顕的および超微形態的研究 : 脛骨骨幹皮質骨について

抄録

Male Wistar rats, 5-week-old, corresponding to humann early childhoodwere given calcium-deficient diet and calucium low-deficient diet for three weeks (group I), for five weeks (group II), and bone debility was induced experimentally. The internal structure of cortex of tibia diaphysis was then investigated. The results were as follows : I. Body weight Body weight of group I and group II decreased in order of the control, calucium low-deficient diet and calcium-deficient diet groups. II. X-ray findings on the bone density Between the cotrol groups and the calcium-deficient diet groups, between the co-ntrol groups and the calcium low-deficient diet groups and between the calcium-deficient diet groups and the calcium low-deficient diet groups, significant difference were found in medial cortex and interosseous cortex of group I and group II, and the cotrol groups, the cotrol groups and the calcium low-deficient diet groups showed higher values (p<0.01). In medial cortex and interosseous cortex, between the cotrol groups and the calcium low-deficient diet groups, significant difference were found between group I and group II and the latter showed higher values (p<0.01). III. Analysis of Ca and P on the X-ray microanalyzer In characteristic X-ray images by plane analysis of Ca and P in same trabecula of medial cortex and interosseous cortex, Ca showed higher density than P in the control group, the calcium-deficient diet groups and the calcium low-deficient diet groups for both group I and group II. IV. Histopathologic findings In the calcium-deficient diet group I as compared with the control groups, osteocytic lacunae and Harversian canal decreased, and Harversian system arranged irregularly were found scattered. In the calcium low-deficient diet groups, Harversian system increased and Harversian canal and the surrounding bone lacunae increased. In the calcium-deficient diet group II as compared with the control groups, innercircumferential lamellae were formed thick and osteocytic lacunae were poor in distribution. In the calcium low-deficient diet groups, outer circumferential lamellae and inner circumferential lamellae were seen clearly and osteocytic lacunae arranged regularly also increased. V. Scanning electron microscopic findings In the calcium-deficient diet group I as compared with the control groups, bone resportion was found extensively and was irregular in shape. The calcium low-deficient diet groups showed denser osseous layers as compared with the calcium-deficient diet groups and collagen fibrills were running regularly and bone lacunae also increased in the bone layer. The calcium-deficient diet group II as compared with that in group I showed decrease in bone construction of trabeculae, and bone resportion was found more extensively and was irregular in shape. The calcium low-deficient diet groups showed many smooth surfaces and bone construction was recognized. VI. Hematologic findings No changes at all were found in any group in electrolytes in serum or chemical examinations and normal values were shown. On the basis of the foregoing findings, durning childhood with calcium insufficiency, for the 11-week-old especially, in the calcium low-deficient diet groups, it was suggested that hormone of bone metabolism had an excellent activity; therefore, it was clear that bone construction had no differece from the control groups.