フローサイトメトリーによるPPD活性化リンパ球の解析

抄録

The present study was undertaken to do phenotype determination of in vitro proliferating lymphocytes by PPD stimulation using combination of monoclonal antibodies and flow- cytometry.
The results obtained were as follows:
1) PPD induced a significant proliferation of activated T cell subsets (Leu4⁺DR⁺cells, IL2-R⁺Leu3⁺cells).
2) PPD also induced a significant increase in Pan T cells (Leu4⁺) and helper T cells (Leu3⁺8^-).
Taken together, these results indicate that PPD induced proliferating lymphocytes belong predominantly to helper T cell subset.
3) The numbers of inducer T cells (Leu3⁺8⁺), suppressor T cells (Leu2⁺15⁺) and cytotoxic T cells (Leu2⁺15^-) were not influenced by PPD-stimulation.
4) However, PPD induced a smaller, but significant proliferation of IL2-R⁺Leu2⁺cells.
5) The number of B cells (Leu4^-DR⁺) tended to increase slightly after PPD-stimulation.