BACTERICIDAL ACTIVITY OF OFLOXACIN AGAINST MYCOBACTERIUM KANSASII

Abstract

Bactericidal activity of ofloxacin against Mycobacterium kansasii was observed inin-vitro experiment. Tested bacteria were suspended to a concentration of one mg wetweight per ml in 10ml of Dubos liquid medium containing no drug or containing 1 or 3kμg/m/l of loxacin and incubated at 37°.
The number of colony-forrming units contained in a 0.02ml-sample of the Dubos liquid medium was counted after incubation for 0, 1, 3 and 7 days. The number of colonies was counted in Ogawa egg medium, to which was inoculateda 0.02ml-sample of dilutions of the medium by a spiral loop that can deliver a 0.02ml-sample by one inoculation. The bactericidal activity of ofloxacin against M. kansasiiappeared after incubation of 3 days or later. This characteristic was different from thebactericidal activity against Mycobacterium tuberculosis, which appeared after incubation of 24 hours.