機械的刺激を受けた筋芽細胞の動的応答

抄録

Experiment of mechanical stimulus was carried out with myoblast cultured on collagen coated silicone sheet subjected to tension for 6 hours to investigate morphological response of the cell. Pictures of its morphological change were taken in computer by CCD camera in every 10 minutes for 8 hours. Both shape index (SI) and cumulative migration for a lot of cells were measured in every 1 hour. SI was increased after tension exposure. In contrast, migration velocity was decreased after tension exposure. Fluid force acts on cytoskeleton during tension exposure as the shape of cell changes. Actin filament as a cytoskeleton and cytosol were modeled to an elastic cylinder and viscous fluid, respectively, to calculate the stress of filament by the fluid-structure interaction analysis. As a result, the maximum stress was 20 Pa, which is consistent with strain velocity provided by the experiment. Taking into account of its Young's Modulus, the rupture of filaments does not cause to increase SI of myoblasts.