Strain distribution in deformed polycrystalline iron plates was investigated using digital image correlation analysis of scanning electron microscope (SEM) images observed before and after tensile tests. Orientation of grains was determined by electron backscattering diagrams (EBSD). Magnitude of strain below 1% could be detected with spatial resolution of 8×8μm2 by the digital image correlation analysis. Inhomogeneous strain distribution was observed in and among grains. It was pointed out that the information of activated slip systems could be obtained by the ratio of strain along the three directions. It was found that the deformation was not always caused by the activation of the slip system of the largest Schmid factor.