This article deals with the sound generating mechanism of ball splines in operation. The results of specific experiments and analyses led to the following conclusions : (1) In the sound wave forms of the ball splines, sound pulses with ball passage period Tp are observed. (2) The main cause of the ball spline sound is the collision between the ball and spline shaft or spline nut at the entrance of the load zone. (3) The sound peaks (the frequencies of peaks are not affected by the linear velocity) are caused by the radial-bending natural vibrations of the spline shaft and spline nut. (4) In the ball splines in operation, the collision between the ball and spline shaft or spline nut produces the radial-bending natural vibrations of the spline shaft and the spline nut, and it results in ball spline sound.