セミクローズド・マイクロチップによるプローブ操作を用いた単一細胞解析への応用(機械力学,計測,自動制御)

抄録

In this paper, we propose a semi-closed microchip which can realize the probe type manipulation in the microchip. The semi-closed microchip has,a bath in the middle of microchannel to allow the insertion of micropipettes into the microchip. The bath is sealed off by thin oil film to prevent the evaporation of the solution in the bath. The seal by the oil film can maintain even if micropipettes are inserted into the bath and pulled out from the bath. The seal by the oil film was evaluated by measuring the weight of the bath after 12/24 hours. To use the semi-closed microchip for cell culture and cell analysis, the exchange of the solution in the semi-closed microchip was demonstrated. The cell fixation with the thermo sensitive gel was also demonstrated by fabricating the ITO electrodes as heaters in the bath. Cell culture and the target cell harvesting were conducted by the semi-closed microchip. The proposed semi-closed microchip can be used for cell culture, cell analysis and cell harvesting under a microscope as the biological applications for single cell analysis.