ENHANCEMENT OF NEURAMINIDASE ACTIVITY WITH INFLUENZA VIRUS BY ANTI-INFLUENZA VIRUS DRUG

抄録

The influence of anti-influenza virus drugs on the neuraminidase activity with three influenza virus strains was examined.
When the allantoic fluid infected with influenza virus strains (A₀/PR8, A₂/Adachi and B/Lee) was treated with 1-adamantanamine hydrochloride (amantadine), the neuraminidase activity was significantly enhanced in all three influenza virus strains. The B/Lee strain exhibited the highest enhancement of neuraminidase activity after 1-adamantanamine hydrochloride treatment.
When purified virus, enzyme source, was treated with various concentrations of 1-adamantanamine hydrochloride, the enhancement of neuraminidase activity with A₀/PR8 strain was observed even at a lower concentration (0.1-400 μg/ml) and the activity further increased with increasing concentration of the drug and reached a plateau at the level of 600-1200 μg/ml. On the other hand, the enhancement of neuraminidase activity with the A₂/Adachi strain was maximum at about 1 μg/ml of 1-adamantanamine hydrochloride.
Treatment with isoquinoline derivatives resulted in the enhancement of neuraminidase activity with the A₀/PR8 and A₂/Adachi strains at a lower concentration, but at a higher concentration, one of the isoquinoline derivatives, UK 2371-01, presented an inhibitory effect.
Our results suggest that the enhancement of neuraminidase activity with the influenza viruses may be a converse action to viral inhibitory activity of anti-influenza virus drugs.