The Keio Journal of Medicine
Online ISSN : 1880-1293
Print ISSN : 0022-9717
ISSN-L : 0022-9717
ELECTRON MICROSCOPIC STUDIES ON THE HUMAN FOLLICULAR OOCYTE CULTURED IN VITRO
KENICHI SEKI
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1975 年 24 巻 2 号 p. 145-157

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Ninety six oocytes from 44 females were cultured in various hours in the chemically defined medium. Ultrastructures of the cultured oocytes were observed by both transmission and scanning electron microscopes. After 40 hours of culture, some of them resumed meiosis and reached to the second meiotic metaphase. The figure of clearly arborizating microvilli were observed. Tubular aggregates existed more in the matured oocyte than the follicular oocyte. The annulate lamellae could not be found in the present study. Other structures such as the granulosa cells, zona pellucida, cytoplasmic organelles and nucleus showed almost the same figures as observed in other studies.
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© by The Keio Journal of Medicine
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