Abstract
1. Naphthol-thionine reaction, Benzidine reaction, Nadi-reaction, Sudan III staining, Naphthol-Sudan staining and Oil red 0 staining have almost the same results when immersed in distilled water with constant temperature each of 60°C, 70°C, 80°C, 90°C and 98°C for 1 minute.
2. And the same results are obtained when immersed in water with temperature of 98°C each for 2, 5, 10 and 30 seconds.
3. These reactions are similarly affected by chemicals.
4. All reactions are noticed negatively or weakly after treating with reducing substance.
5. As above-mentioned, the temperature and time to inhibit these reactions have a mutual relation. Although there are slight differences between them, it can not be considered that these reactions are different in the nature from each other.
And considering the fact that the granules in leukocytes can be stained with indophenol blue, Sudan III and thionine by adding naphthol, the acting point of acid or heat is suspected to be the part which has no connection with enzyme and on which naphthol combines with granules.
It could be concluded that these reactions are resulted from the special affinity of ISS to naphthol and they are, identical in the nature.
