組織培養における微電流の支持組織に及ぼす影響について
1960 年 10 巻 5 号 p. 510-528
詳細
1960 年 10 巻 5 号 p. 510-528
The author wanted to see what effect the minute electric current generated in the living body would exert on the growth of fibroblasts and bone. For this purpose, the femur and fibroblasts of the heart from the chick embryo were cultured. A modification of the Carrel's bottle was used for the convenience of applying electric current.
The growth of 3rd serial culture of the fibroblasts was inhibited by a current of 7.5, 5.0 and 2.1 μA in varying degrees according to the current strength, accelerated by 1.2 μA, and slightly inhibited by 0.1 μA.
In passing a 5.0 μA current, the growth was accelerated toward the anode, exhibiting remarkable “polarity”. On the cathodic side, however, only a trace of “polarity” was recognized. In the case of 2.1 μA, remarkable polarity was observed toward both poles. Especially toward the anodic side, the end of the tissue was pointed owing to stronger polarity, the tissue consequently assuming an oval form.
The growth of the femur was remarkably inhibited by the application of 7.5 μA current, and slightly inhibited by 5.0, 2.1, 1.2, and 0.1 μA, without showing change according to current strength.
