北関東医学
Online ISSN : 1883-6135
Print ISSN : 0023-1908
ISSN-L : 0023-1908
型特異性分解酵素によるヒト血球のAおよびB 型物質の研究
藤沢 敬一古川 研赤羽 重吾
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1963 年 13 巻 1 号 p. 19-39

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1. Human erythrocytes, stroma, glycolipid (Fr. I and mixture of Fr. II-IV), and residue after extraction of the stroma (Fr. V) contain galactose, glucose, mannose, fucose, N-acetylgalactosamine and N-acetylglucosamine. Sialic acid and fast moving components are also contained in the glycolipid fractions, except in Fr. I.
2. Preparation of A-decomposing enzyme from Cl. tertium, abolishes the A-specific activity of human A substance from erythrocytes, liberating from it not only monosaccharides, galactose and N-acetyl galactosamine, but also a disaccharide consisting of galactose and N-acetylgalactosamine, and sialic acid.
3. Preparation of B-decomposing enzyme from Cl. maebashi, abolishes the B-specific activity of human B substance from erythrocytes, liberating from it only galactose, as a monosaccharide.
4. A-combining sites on an erythrocyte are estimated to 7.3 × 106, and B-combining sites on an erythrocyte 7 × 106.
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