性ホルモン投与によるラットの水分代謝及び電解質の変化に関する研究

抄録

Water and electrolyte metabolism in rats were observed in each estrus cycle and after administration of sexual hormons, and the following results were obtained.
1 Circulating serum was significanty increased in estrus II. extracellular fluid in estrus I, and serum sodium in estrus I and II, while serum potassium and total serum protein were both significantly decreased in estrus II. (classification of estrus by Koyama and Nakao, 1954)
2 When rats were injected with PMS for 5 days, circulating serum was significantly decreases with daily dose of 100 i. u. and 500 i. u., while extracellular fluid was significantly increased with 100 i. u. and 500 i. u., serum sodium with 10 i. u., 100 i. u. and 500 i. u. and serum potassium and total serum protein with 10 i. u. and 500 i. u., There was no quantitative change in serum chloride and sodium chloride.
3 When rats were injected with HCG for 5 days, circulating serum was significantly decreased with daily dose of 10 i. u., 100 i. u. and 500 i. u., while extracellular fluid was significantly increased with. 10 i. u. and 100 i. u., serum sodium with. 10 i. u. and 100 i. u., serum potassium with 10 i. u. and 500 i. u., and total serum protein with 100 i. u. and 500 i. u., There was no quantative change in serum chloride and sodium chloride.
4 Rats were injected with PMS for 5 days and then with HCG for the succeding 5 days in the following daily doses. In group A. 10 i. u. each, in group B, 100 i, u. each, and group C, 500 i, u, each. Circulating serum was significantly decreased in all the groups, and so was extracellulr fluid in group A, while serum sodium and potassium were significantly increased in all the groups, and so were serum chloride and sodium chloride in group A, and total serum protein in all the groups.
5 When rats were injected with estradiol benzoate for 5 days, circulating serum was significantly decreased with daily dose of 0.02mg and 2mg, while extracellular fluid was significantly increased with 0.2mg, serum potasium with 0.2mg, and 0.4mg, serum chloride and sodium chloride with 0.2mg and 0.4mg, serum sodium and total serum protein with 0.02mg, 0.02mg and 0.04mg.
6 When rats were injected with progesterone for 5 days, circulating serum was significantly decreased with daily dose of 1 mg and 10mg, while serum sodium and potassium were significantly increased with 1 mg, 2 mg and 10mg, and serum chloride and sodium chloride with 1 mg and 2 mg. There was quantative change in extracellular fluid.
7 Estradiol benzoate and progesterone were jointly given for 5 days in the following dose, In group A, 0.02mg and 1 mg, in groupe B, 0.2mg and 2 mg, and in group C, 0.4mg and 10mg, respectively. Circulating serum was significantly decreased in group A and significantly increased in group C. Those were no remarkable change in extracelluar fluid, and serum chloride and sodium chloride. Serum sodium was significantly increased in group B and C, and total serum protein in all the groups. Whereas serum potassium was significantly decreased in group A, and significantly increased in group B.
8 Rats were injected with estradiol benzoate for 5 days and then with progesterone for the succeeding 5 days in the following doses. In group A, 0. 02mg and 1 mg; in group B, 0.2mg and 2 mg, in group C, 0.4 mg and 10 mg, respectively. Circulating serum was significantly decreased in group A, B and C, and serum potassium in group A, while serum sodium was significantly increased in group B and total serum protein in all the groups. There were no quantitative changes in extracellular fluid and serum chloride and sodium chloride.