6-メルカプトプリン耐性マウス白血病細胞 (L1210)
二つのタイプの耐性株
1979 年 29 巻 1 号 p. 13-20
詳細
1979 年 29 巻 1 号 p. 13-20
Mouse leukemia cell (L1210) is sensitive to 6-mercaptopurine (6-MP) and does not usually grow in medium containing 0.1 μg/ml of 6-MP. However, cells which could grow in medium containing 0.3 μ/ml of 6-MP were easily obtained at a rate of about 10-5.
There were two types of 6-MP resistant strains thus obtained in 0.3 pig/ml of 6-MP; the one was low-resistant strain which could grow in 1 μg/ml of 6-MP but could not in 3 μg/ml, and the other was high-resistant strain which could grow even in 30 μg/ml of 6-MP. Most (96.1 %) of the resistant cells obtained in medium containing 0.3 μg/ml of 6-MP belong to high resistant strain and 3.9% to low-resistant one.
The resistance mechamisms of these two strains were not clarified but might be different from each other by the following results;
1) Low-resistant strain could grow in HAT medium but high-resistant strain could not.
2) The sensitivity of low-resistant strain to 6-MP was increased by the treatment with Levamisole, whereas no change was found in high-resistant strain.
3) Reversion to sensitive cell could be observed in the low-resistant strain but not in high-resistant one.
4) Only high-resistant cell could obtained from the revertant cell but no low-resistant cell could be obtained.
