低温処理による培養熱帯熱マラリア原虫の同調
1985 年 35 巻 4 号 p. 383-390
詳細
1985 年 35 巻 4 号 p. 383-390
A simple, highly reproducible method of synchronizing cultured Plasmodium (P) falciparum was studied. When culture of P. falciparum was kept at 0°C for 48 hours, the parasites showed a stepwise multiplication curve in 37°C incubation, while nontreated parasites manifested a linear continuous propagation pattern. Morphological observation revealed that parasites grew synchronously after cold treatment. Only parasites at the stage of the young ring form survived after 0°C exposure, and this caused homogeneity of the developmental stage of treated parasites. Syn-chronicity of the treated parasites was also confirmed by boosted DNA and protein syntheses when parasites reached the late trophozoite stage. Several advantages over other synchronization methods are discussed.
