ビオチン標識プローブを用いた In situ Hybridization によるヒト剖検組織における単純ヘルペスウィルスゲノムの証明
1990 年 40 巻 3 号 p. 297-301
詳細
1990 年 40 巻 3 号 p. 297-301
DNA : DNA in situ hybridization (ISH) using a biotinylated cDNA probe was carried out to investigate the presence of herpes simplex virus (HSV) genome in formalin-fixed, paraffin-embedded tissue sections from human autopsy cases. In cases of HSV infection, HSV DNA was clearly identified by ISH in the nuclei of cells with Cowdry type A or full type inclusions. No background staining was observed. None of the control sections from esophagus or brain from a case of necrotizing encephalitis hybridized with the HSV DNA probe. This study suggests that ISH using a biotin labeled HSV DNA probe is a specific and convenient method for confirming the diagnosis of HSV infection.
