Abstract
Analysis of human red blood cells surface properties and evaluation of the interactions among red blood cells were done to establish the mechanism of the enzyme method. Electrophoretic mobilities of intact and enzyme-treated red blood cells were analyzed with soft particle theories; one based on the diffuse models could provide further information about the properties of red blood cell surfaces. Therefore, the interaction energies between two red blood cells were evaluated and the closest distances between them were evaluated to decide the agglutinability of cells with a IgG molecule. It was concluded that thinning of the surface layer thickness plays more important role in the enzyme method than reducing the surface layer charge densities.
