Abstract
Endotoxins are a major component of the outer membrane of Gram-negative bacteria. Since they lead to multiple pathophysiological effects, efficient removal of endotoxins from biological and pharmaceutical products is essential. We successfully prepared polymer hydrogel beads with endotoxin adsorption sites by applying a molecular imprinting technique, using a triazine derivative as a functional monomer, that was polymerized in an inverse-phase suspension. The gel beads showed a high adsorption capacity for endotoxin, and exhibited a preferential binding for endotoxin rather than BSA. These results suggest that the gel beads can be employed for an endotoxin adsorbent.
